基本简介

编号 AMS-91-07

拉丁菌名 Dipariforma spartinacae H.L.Zhou, Y.G.Zhou et C.S.Xiao nov. sp.

中文菌名 产琥珀酸梭菌

定名人 谭蓓英 王大耜

Nomencla Zhou Huiling, Zhou Yuguang, and Xiao Changsong

发表文章 一个嗜热分解纤维素的梭菌新种的分离和鉴定

Article A New Gwnus of Budding and Appendaged Bacteria

作者信息

作者 谭蓓英 王大耜

Author Zhou Huiling, Zhou Yuguang, and Xiao Changsong

作者单位 中国科学院微生物研究所细菌分类室, 北京 100080

内容

本文描述了一个严格 厌氧, 形成芽孢, 嗜热, 分解纤维素, 糖和蛋白质的梭菌 新种. 这个种的两株菌 (H11, H12) 是从奶牛场的青贮饲 料(山草)中分离出来的. 在PYC液体培养基中细胞杆状 , 0.3-0.6 X 1.5-5.5 micrometer, 单生或成对, 很少成 链. 革兰氏阴性, 能运动, 有周年鞭毛. 芽孢卵至球形, 深层菌呈双凸镜状,湿润, 浅黄色, 直径1－2mm, 边缘完 整. 生长的量适条件是 50-53'C 和 pH7.5. 所需要的生 长因子是生物素, 维生素B4, B12和对氨基苯甲酸. 发酵 纤维素或纤维二糖产生琥珀酸,乙酸, 乙醇, 氢和二氧化 碳. DNA的 G+C 含量是 38.8 mol%. 参考这个种在非挥发 酸中的主要产物是琥珀酸的特性, 将它定名为产琥珀酸梭 菌 (Clostridium succinogenum Tan & Wang sp. nov.) .

Abstract A Strain of oxidative Gram negative bacteria, No.4088, was isolated from the root of Sparina anglica grown at the beach of Rudong County, Jiangsu Province.The cells of the strain No.4088 are rod or ovoid shaped, 0.8-1.0 by 1.1-1.5 micrometer, with capsule, non-motile. The grenules of poly-B-hydroxybutyrate andyellow pigment of B-carotin type are produced. Strain No.4088 multiplies with two forms of budding.The one is buds formed primarily by swelling of the prosthecae (hyphae). The other is with yeast-like form; about 20% buds sprout from mother cells directly.These two types of budding may appear on the same cell Strain No.4088 is an organism with respiratory metabolism, never fermentative, aerobic, microaerophilicunder combined nitrogen-free codition. Chemoorganotrophilic, no acid is produced from sugars and alcohols. Glucose, xylose, maltose, acetate, citrate,succinate, malate, a-ketoglutarate, malonate, arginine and casein are utilized as the sole carbon and energy sources. Methanol, formate, propinate, butyrate,iso-butyrate, valerate, iso-valerate, caprate, fumarate, huppurate, aminoacetate, proline, 2,3-butylene glycol, or betaine is no utilized. Oxidase and catalaseare positive. MR and VP are negative. Hydrogen sulfide is not produced. Nitrate is reduced. Litmus milk becomes alkaline. Esculin, starch or gelatinis not hydrolized. Lysine decarboxylase and arginine decarboxylase are positive and ornithine decarboxylase, DNase, and lipase, urease, arginedihydrolase, phenylalanine deaminase are negative. Free nitrogen can be fixed. Lron and manganese can be deposited. Growth occurs in medium containingNaCl not more than 1.5%. Growth temperature: 20-35'C, 30'C is optimum. Growth pH 6.0-9.0, optimum is ph7.5. Guanine and cytidine contents in DNA is 70.1-70.2 mol%.

Budding and appendaged bacteria multiply with three types: binnary,fission, budding at tips of prostecae (hyphae) or budding from the mother cell directly.But no genus posseses both tips of multiplication as strain No.4088, which buds as at tips of posthecae and from mother cell directly.

Pedomicrobium buds at the tips or laterally at the posthecae. Daughter cell are flagellated.

Hyphomonas buds are produced at tips of polar prosthecae (hyphae). Prosthecae are nonseparate and arrely branch under normal growth conditions.Motile by means of a single polar or lateral flagellum located on developing buds or young daughter cell. Amino acids are required.

Hyphomicrobium daughter cell formation by a buuding process at one prothecae, mature buds become motile as swarmers. Poly-B-hydrobutyrateis stored by most cells usually at a district cell pole. The prothecae are not separate.