基 本 信 息

编号 AMS-91-06

拉丁菌名 Dipariforma H.L.Zhou, Y.G.Zhou et C.S.Xiao gen. nov.

中文菌名 南昌小链孢菌

定名人 余名仑

Nomencla Zhou Huiling, Zhou Yuguang, and Xiao Changsong

发表文章 小链孢菌属的一个新种

Article A New Gwnus of Budding and Appendaged Bacteria

作者信息

作者 余名仑

Author Zhou Huiling, Zhou Yuguang, and Xiao Changsong

作者单位 江西大学生物系, 南昌 330047

摘 要

小链孢菌属 (Microstreptospora) 是放线菌目 (Actinomycetales) 的新属. 至今仅发表过一个种. 我们于1983年分离到 一株放线菌, 经鉴定认为是该属的一个新种.

Abstract A Strain of oxidative Gram negative bacteria, No.4088, was isolated from the root of Sparina anglica grown at the beach of Rudong County, Jiangsu Province.The cells of the strain No.4088 are rod or ovoid shaped, 0.8-1.0 by 1.1-1.5 micrometer, with capsule, non-motile. The grenules of poly-B-hydroxybutyrateand yellow pigment of B-carotin type are produced. Strain No.4088 multiplies with two forms of budding.The one is buds formed primarily by swelling of the prosthecae (hyphae). The other is with yeast-like form; about 20% buds sprout from mother cells directly.These two types of budding may appear on the same cell Strain No.4088 is an organism with respiratory metabolism, never fermentative, aerobic, microaerophilicunder combined nitrogen-free codition. Chemoorganotrophilic, no acid is produced from sugars and alcohols. Glucose, xylose, maltose, acetate, citrate,succinate, malate, a-ketoglutarate, malonate, arginine and casein are utilized as the sole carbon and energy sources. Methanol, formate, propinate, butyrate,iso-butyrate, valerate, iso-valerate, caprate, fumarate, huppurate, aminoacetate, proline, 2,3-butylene glycol, or betaine is no utilized. Oxidase and catalaseare positive. MR and VP are negative. Hydrogen sulfide is not produced. Nitrate is reduced. Litmus milk becomes alkaline. Esculin, starch or gelatinis not hydrolized. Lysine decarboxylase and arginine decarboxylase are positive and ornithine decarboxylase, DNase, and lipase, urease, arginedihydrolase, phenylalanine deaminase are negative. Free nitrogen can be fixed. Lron and manganese can be deposited. Growth occurs in medium containingNaCl not more than 1.5%. Growth temperature: 20-35'C, 30'C is optimum. Growth pH 6.0-9.0, optimum is ph7.5. Guanine and cytidine contents in DNA is 70.1-70.2 mol%.

Budding and appendaged bacteria multiply with three types: binnary,fission, budding at tips of prostecae (hyphae) or budding from the mother cell directly.But no genus posseses both tips of multiplication as strain No.4088, which buds as at tips of posthecae and from mother cell directly.

Pedomicrobium buds at the tips or laterally at the posthecae. Daughter cell are flagellated.

Hyphomonas buds are produced at tips of polar prosthecae (hyphae). Prosthecae are nonseparate and arrely branch under normal growth conditions.Motile by means of a single polar or lateral flagellum located on developing buds or young daughter cell. Amino acids are required.

Hyphomicrobium daughter cell formation by a buuding process at one prothecae, mature buds become motile as swarmers. Poly-B-hydrobutyrateis stored by most cells usually at a district cell pole. The prothecae are not separate.